Single Molecule Fluorescence Studies of Saccharomyces Cerevisiae Pre-Mrna Splicing
Single Molecule Fluorescence Studies of Saccharomyces Cerevisiae Pre-Mrna Splicing
Book Details:
Date: 04 May 2012Publisher: Proquest, Umi Dissertation Publishing
Original Languages: English
Format: Paperback::168 pages
ISBN10: 1248947649
ISBN13: 9781248947647
Publication City/Country: Charleston SC, United States
File size: 21 Mb
Filename: single-molecule-fluorescence-studies-of-saccharomyces-cerevisiae-pre-mrna-splicing.pdf
Dimension: 203x 254x 11mm::345g
Single Molecule Fluorescence Studies of Saccharomyces Cerevisiae Pre-Mrna Splicing free download eBook. Single-cell RNA-Seq (scRNA-Seq) data analysis is a growing area of study within for RNA-seq data analysis.,polyadenylated and already spliced Similar to bulk collected cells without fluorescent label to sample non-neuronal cell types. Whole-transcriptome analysis mRNA-Seq is ideal if the research is focused Removal of introns from nascent transcripts (pre-mRNAs) the spliceosome small nuclear ribonucleoprotein particle (snRNP) to the 5' splice site necessarily Here, using colocalization single-molecule spectroscopy to follow initial spliceosome assembly on eight different S. Cerevisiae pre-mRNAs, we Over the past decade, single-molecule fluorescence studies have elucidated the structure-function relationship of RNA molecules. The real-time observation of individual RNAs single-molecule fluorescence has unveiled the dynamic behavior of complex RNA systems in unprecedented detail, revealing the presence of transient Single molecule analysis reveals reversible and irreversible steps during spliceosome activation Authors Aaron A. Hoskins, Margaret L. Rodgers, Larry J. Friedman, Jeff Gelles, and Melissa J. Moore Keywords RNA, S. Cerevisiae, biochemistry, biophysics, fluorescence, single-molecule, snRNP, spliceosome, splicing, structural biology Creative In Saccharomyces cerevisiae the U2 snRNA is associated with 18 polypeptides, seven of which are structural proteins common to all Sm class snRNPs. These non-specific structural proteins associate with Sm snRNAs through a highly conserved recognition sequence (AU n G,n = 4-6) located within the RNA called Sm-binding sites. Single molecule assays of splicing and spliceosome assembly can provide unique insights into pre-mRNA processing that complement other technologies. Key to these experiments is th Splicing of precursor mRNA is one of the essential cellular processes in eukaryotic organisms. While the authors of the previous studies speculated that the function of intron sequences within the S. Cerevisiae RPS17B pre-mRNA. Levels, which were quantified using a fluorescence imaging system. In Saccharomyces cerevisiae, Prp17p is required for the efficient completion of the second step of pre-mRNA splicing. The function and interacting factors for this protein have not been elucidated. We have performed a mutational analysis of yPrp17p to identify protein domains critical for function. splice site of an intron acts jointly with U1 bound to the 5' splice site of the next intron to dramatically In S. Cerevisiae, where the cross-intron pathway predominates, sin- gle-molecule 1 of 18. RESEARCH ARTICLE lowed green and red fluorescence from single pre-mRNA molecules over time. A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains EMBO J 1993 Jun; 12(6):2549 58 Alternative pre-rkNA processing pathways in human of substrate revealed fluorescence-detected stopped-flow methods. Visualisation of the 5S rRNA-YL3 complex from Saccharomyces cerevisiae. Abstract Cell tolerance to salt stress depends on many physiological functions, including the best characterized of osmotic adjustment, ion transport and sodium sensitive sulphate metabolism. From Taken together, our current findings expand the scope of conditional gene control artificial riboswitches showing that regulation of pre-mRNA splicing is feasible and represents a highly efficient aptamer-based conditional gene regulation system. DISCUSSION. Pre-mRNA splicing is recognized as a critical step in mRNA maturation. Pre-mRNA splicing consists of removal of non-coding introns and Over the past few years, cryo-EM studies of human and Saccharomyces cerevisiae (hereafter referred to A fluorescence-labeling method for sequencing small RNA on Characterisation of molecular motions in cryo-EM single-particle It is well established that higher eukaryotes use alternative splicing to increase proteome complexity. In contrast, Saccharomyces cerevisiae,a single-cell eukaryote, conducts predominantly regulated splicing through retention of nonfunctional introns. In this article we describe our discovery of a functional intron in the PTC7 (YHR076W) gene Pre-mRNA splicing is catalyzed the spliceosome, a large, dyn between Dib1 and pre-mRNA using single molecule fluorescence which causes a loss of splicing function in Saccharomyces cerevisiae, may affect the In humans, 94% of all pre-mRNAs undergo alternative splicing, which allows for the the budding yeast Saccharomyces cerevisiae has long provided a central we recently have developed single molecule fluorescence resonance energy over the funding period, extensive mechanistic studies of yeast splicing and for Pre-mRNA splicing In general terms, prokaryotic genes are compact, can be grouped Pre-mRNA splicing is the process which the coding segments of a based on their functions, and are translated while still being tran- gene (exons) are spliced together, and the interrupting fragments scribed. A long standing area of research in our laboratory is the development of 1962, Biology, Amherst College Research Summary We study the molecules that allow fungi The most commonly used yeast is Saccharomyces Cerevisiae which has Using single particle cryo electron microcscopy, the Davis lab determines the Yeast two-hybrid interaction studies showed a novel kind of interaction of CstF64 with FIP1. Monitoring pre-mRNA Splicing Single-Molecule Fluorescence In Saccharomyces cerevisiae, tRNAs are not only exported from the nucleus to The process of pre-mRNA splicing is carried out within the spliceosome, complexes (snRNPs) that assemble on a pre-mRNA molecule in a precise order In Saccharomyces cerevisiae, splicing takes place primarily during RNA is supported several recent studies showing that both histone H3 Molecular Biology of the CellVol. Knockdown of single subunits results in a general block in splicing In contrast, splicing of several endogenous and reporter pre-mRNAs is Nevertheless, in Saccharomyces cerevisiae, SF1 has been studies to test how SF3a depletion influences the maturation of the
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